首页> 外文OA文献 >Pseudopleurochloris antarctica gen. et sp. nov. a new coccoid xanthophycean from pack-ice of Wood Bay (Ross Sea, Antarctica): ultrastructure, pigments and 18S rRNA gene sequence

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Pseudopleurochloris antarctica gen. et sp. nov. a new coccoid xanthophycean from pack-ice of Wood Bay (Ross Sea, Antarctica): ultrastructure, pigments and 18S rRNA gene sequence

机译：南极拟绿假单胞菌。等。十一月Wood Bay（南极洲罗斯海）pack-ice的一种新的球形球藻类黄藻：超微结构，色素和18S rRNA基因序列

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A microalga was isolated from the pack-ice of Wood Bay (Antarctica) during austral summer 1993–4. By comparison with an authentic strain obtained from Sammlung von Algenkulturen at the University of Göttingen (SAG 860–3), its ultrastructural details and pigment composition were very similar to those of Pleurochloris meiringensis. However, sequence analysis of the entire 18S rRNA gene revealed substantial genetic divergence (57 nucleotide substitutions and 19 insertions/deletions) between SAG 860–3 and the Antarctic isolate. Phylogenetic analysis of complete 18S rRNA gene sequences from 18 microalgal species, including the two strains studied, rejected the hypothesis that the Antarctic isolate is the sister species to Pleurochloris meiringensis. Molecular evidence indicates that the Antarctic isolate is the sister group of a clade that comprises two other representatives of the Xanthophyceae (synonym Tribophyceae): Pleurochloris meiringensis and Botrydiopsis intercedens. This leads us to conclude that this microalga is a new species belonging to a new genus and the name Pseudopleurochloris antarctica is proposed.

机译：在1993-4夏季，从伍德湾（南极洲）的冰袋中分离出了微藻。与从哥廷根大学的Sammlung von Algenkulturen获得的真实菌株进行比较（SAG 860-3），其超微结构细节和色素组成与白灵菇相似。但是，对整个18S rRNA基因的序列分析显示，SAG 860-3与南极分离株之间存在显着的遗传差异（57个核苷酸取代和19个插入/缺失）。从18个微藻物种（包括所研究的两个菌株）对完整的18S rRNA基因序列进行系统进化分析，拒绝了南极分离株是Meiringensis的姊妹物种的假说。分子证据表明，南极分离株是进化枝的姊妹群，该进化枝包含叶黄藻科的两个其他代表（同义词Tribophyceae）：绿脓杆菌和间孢菌。这使我们得出结论，该微藻是属于新属的一个新物种，并提出了南极假胸藻的名称。

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ANDREOLI C; MORO I; LA ROCCA N; RIGONI F; DALLA VALLE L; BARGELLONI L;
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Pseudopleurochloris antarctica gen. et sp. nov. a new coccoid xanthophycean from pack-ice of Wood Bay (Ross Sea, Antarctica): ultrastructure, pigments and 18S rRNA gene sequence

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